Glossary entry (derived from question below)
English term or phrase:
Rolling circle amplification
Vietnamese translation:
kỹ thuật khuếch đại xoay vòng
Added to glossary by
Hien Luu
Oct 3, 2023 22:45
8 mos ago
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English term
Rolling circle amplification
English to Vietnamese
Tech/Engineering
Agriculture
Context:
Rolling circle amplification (RCA) is a method of isothermal amplification of circular DNA molecules. RCA assay involves DNA amplification using a DNA ɸ polymerase with strand displacement activity to extend a single or multiple primers annealed to a circular DNA template. The strand displacement activity allows the newly synthesized DNA template to displace the previously generated DNA molecule releasing single-stranded DNA (ssDNA). This enzymatic process of primer extension combined with DNA strand displacement generates a long single-stranded DNA containing a repeated sequence complementary to the circular DNA template. The method is successfully used for the detection of many circular single- and double-stranded DNA viruses (begomovirus, badnavirus) infecting different crops.
Rolling circle amplification (RCA) is a method of isothermal amplification of circular DNA molecules. RCA assay involves DNA amplification using a DNA ɸ polymerase with strand displacement activity to extend a single or multiple primers annealed to a circular DNA template. The strand displacement activity allows the newly synthesized DNA template to displace the previously generated DNA molecule releasing single-stranded DNA (ssDNA). This enzymatic process of primer extension combined with DNA strand displacement generates a long single-stranded DNA containing a repeated sequence complementary to the circular DNA template. The method is successfully used for the detection of many circular single- and double-stranded DNA viruses (begomovirus, badnavirus) infecting different crops.
Proposed translations
(Vietnamese)
5 | kỹ thuật khuếch đại xoay vòng | Tung Nguyen |
4 | khuếch đại vòng tròn lăn | Kiet Bach |
Proposed translations
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kỹ thuật khuếch đại xoay vòng
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3 hrs
khuếch đại vòng tròn lăn
Một số chiến lược khuếch đại đẵng nhiệt, bao gồm ... khuếch đại vòng tròn lăn, ...https://thuysanvietnam.com.vn/cong-nghe-phat-hien-virus-o-to...
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